Please Note: This is not a design tool!
This tool is made to help you find matching primers and probes for TaqMan assays within your specified target sequence. By providing the exact (single isotope) masses of both primers and the probe, the chemical modifications of the probe, the length of the amplicon, and the reference sequence (RefSeq), you can easily identify compatible components of your assay. Ensure all details are accurate for optimal results.
You can test this tool using this example dataset for the Actin Beta TaqMan assay Hs99999903_m1 from Thermo Fisher. Please note, the output of this tool can sometimes be ambiguous, providing several probes with the same mass. For this specific example, the actual sequences can be found in this publication.
Mass of Primer 1
Mass of Primer 2
Mass of Probe
Dye-Quencher
Amplicon size
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